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Sigma-E is required for the production of the antibiotic actinomycin in Streptomyces antibioticus
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posted on 2023-06-08, 05:08 authored by George H Jones, Mark PagetMark Paget, Leony Chamberlin, Mark J ButtnerThe phsA gene encodes phenoxazinone synthase (PHS), which catalyses the penultimate step in the pathway for actinomycin biosynthesis in Streptomyces antibioticus. The phsA promoter strikingly resembles a putative Streptomyces s(E) cognate promoter, and purified Es(E) holoenzyme transcribed the phsA promoter in vitro. However, the phsA promoter was still active in an S. antibioticus sigE null mutant and the level of PHS activity was unaffected. Despite this, disruption of sigE blocked actinomycin production completely. The loss of actinomycin production correlated with a 10-fold decrease in the activity of actinomycin synthetase I, the enzyme which catalyses the activation of the precursor of the actinomycin chromophore.
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Publication status
- Published
Journal
Molecular MicrobiologyISSN
0950382XIssue
1Volume
23Page range
169-178Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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