A Pathway of Double-Strand Break Rejoining Dependent upon ATM, Artemis, and Proteins Locating to ¿-H2AX Foci

Riballo, Enriqueta, Kühne, Martin, Rief, Nicole, Doherty, Aidan, Smith, Graeme C M, Recio, Marı́a-José, Reis, Caroline, Dahm, Kirsten, Fricke, Andreas, Krempler, Andrea, Parker, Antony R, Jackson, Stephen P, Gennery, Andrew, Jeggo, Penny A and Löbrich, Markus (2004) A Pathway of Double-Strand Break Rejoining Dependent upon ATM, Artemis, and Proteins Locating to ¿-H2AX Foci. Molecular Cell, 16 (5). pp. 715-724. ISSN 1097-2765

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Abstract

The hereditary disorder ataxia telangiectasia (A-T) is associated with striking cellular radiosensitivity that cannot be attributed to the characterized cell cycle checkpoint defects. By epistasis analysis, we show that ataxia telangiectasia mutated protein (ATM) and Artemis, the protein defective in patients with RS-SCID, function in a common double-strand break (DSB) repair pathway that also requires H2AX, 53BP1, Nbs1, Mre11, and DNA-PK. We show that radiation-induced Artemis hyperphosphorylation is ATM dependent. The DSB repair process requires Artemis nuclease activity and rejoins approximately 10% of radiation-induced DSBs. Our findings are consistent with a model in which ATM is required for Artemis-dependent processing of double-stranded ends with damaged termini. We demonstrate that Artemis is a downstream component of the ATM signaling pathway required uniquely for the DSB repair function but dispensable for ATM-dependent cell cycle checkpoint arrest. The significant radiosensitivity of Artemis-deficient cells demonstrates the importance of this component of DSB repair to survival.

Item Type: Article
Additional Information: Designed project. Work done in PJ lab. Wrote paper. Desrcibes discovery of a pathway of double strand break repair defective in several disorders.
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Queti Riballo
Date Deposited: 06 Feb 2012 20:00
Last Modified: 30 Mar 2012 13:54
URI: http://sro.sussex.ac.uk/id/eprint/23482
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