Investigation of the Potential of the Dissolution Dynamic Nuclear Polarization Method for General Sensitivity Enhancement in Small-Molecule NMR Spectroscopy

Day, I J, Mitchell, J C, Snowden, M J and Davis, A L (2008) Investigation of the Potential of the Dissolution Dynamic Nuclear Polarization Method for General Sensitivity Enhancement in Small-Molecule NMR Spectroscopy. Applied Magnetic Resonance, 34 (3-4). pp. 453-460. ISSN 0937-9347

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Abstract

We report results of applying a commercial implementation of the dissolution dynamic nuclear polarization (DNP) methodology developed by K. G. Golman et al. to a range of molecular species in the mass range of 100400 Da. The molecules are typical of those that might be encountered in natural product chemistry or pharmaceutical analysis. Using an experimental protocol previously reported, in combination with the ERETIC method for generating a reference signal for estimation of concentra- tions, we determine the signal enhancement and high-field, liquid-state T1 values for many of the carbon atoms in the six species studied. The results presented in this work suggest that the measured varia- tion in nuclear magnetic resonance enhancements within a given molecule, arising from the dissolu- tion DNP method, is accounted for principally by relaxation of 13C atoms towards thermal polariza- tion values in the liquid state. We conclude that dissolution DNP will be able to be employed for a wide range of chemical species, provided that the total time taken for dissolution and transfer of so- lutions is comparable to, or shorter than, the high-field, liquid-state T1 values in the species being studied.

Item Type: Article
Schools and Departments: School of Life Sciences > Chemistry
Depositing User: Iain Day
Date Deposited: 06 Feb 2012 20:00
Last Modified: 26 Mar 2012 11:47
URI: http://sro.sussex.ac.uk/id/eprint/23465
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