Suppression of nitric oxide (NO)-dependent behavior by double-stranded RNA-mediated silencing of a neuronal NO synthase gene

Korneev, Sergei A, Kemenes, Ildikó, Straub, Volko, Staras, Kevin, Korneeva, Elena I, Kemenes, György, Benjamin, Paul R and O’Shea, Michael (2002) Suppression of nitric oxide (NO)-dependent behavior by double-stranded RNA-mediated silencing of a neuronal NO synthase gene. Journal of Neuroscience, 22 (11). RC227. ISSN 0270-6474

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Abstract

We have used double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) to disrupt neuronal nitric oxide (NO) synthase (nNOS) gene function in the snail Lymnaea stagnalis and have detected a specific behavioral phenotype. The injection of whole animals with synthetic dsRNA molecules targeted to the nNOS-encoding mRNA reduces feeding behavior in vivo and fictive feeding in vitro and interferes with NO synthesis by the CNS. By showing that synthetic dsRNA targeted to the nNOS mRNA causes a significant and long-lasting reduction in the levels of Lym-nNOS mRNA, we verify that specific RNAi has occurred. Importantly, our results establish that the expression of nNOS gene is essential for normal feeding behavior. They also show that dsRNA can be used in the investigation of functional gene expression in the context of whole animal behavior, regardless of the availability of targeted mutation technologies.

Item Type: Article
Keywords: nNOS; dsRNA; RNAi; gene silencing; feeding behavior; Lymnaea stagnalis
Schools and Departments: School of Life Sciences > Neuroscience
Depositing User: Sergei Korneev
Date Deposited: 06 Feb 2012 19:58
Last Modified: 07 Mar 2017 07:55
URI: http://sro.sussex.ac.uk/id/eprint/23262

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