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Expression of the Pax2 Transcription Factor is Associated with Vestibular Phenotype in the Avian Inner Ear
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posted on 2023-06-08, 00:29 authored by M E Warchol, Guy RichardsonThe paired-domain transcription factor Pax2 is involved in many facets of inner ear development, hot relatively little is known about the expression or function of Pax2 in the mature ear. In this study, we have used immunohistochemical methods to characterize the expression patterns of Pax2 in the sensory organs of inner ears from posthatch chicks. Immunoreactivity for Pax2 was observed in the nuclei of most hair cells and supporting cells in the vestibular organs. In contrast, Pax2 expression in the chick cochlea was limited to hair cells located in the very distal (low frequency) region. We then used organotypic cultures of the chick utricle to examine changes in Pax2 expression in response to ototoxic in, jury and during hair cell regeneration. Treatment with streptomycin resulted in the loss or most Pax2 immunoreactivity from the lumenal (hair cell) stratum of the utricle. During the early phases of regeneration, moderate Pax2 expression was maintained in the nuclei of proliferating supporting cells. Expression of Pax2 in the hair cell stratum recovered in parallel with hair cell regeneration. The results indicate that Pax2 continues to he expressed in the mature avian ear, and that its expression pattern is correlated with a vestibular phenotype.
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Publication status
- Published
Journal
Developmental NeurobiologyISSN
1932-8451Publisher
Wiley-BlackwellExternal DOI
Issue
2-3Volume
69Page range
191-202Pages
12.0Department affiliated with
- Neuroscience Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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