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Translesion DNA synthesis-assisted non-homologous end-joining of complex double-strand breaks prevents loss of DNA sequences in mammalian cells

journal contribution
posted on 2023-06-07, 15:12 authored by Shay Covo, Jean-Pierre de Villartay, Penny Jeggo, Zvi Livneh
Double strand breaks (DSB) are severe DNA lesions, and if not properly repaired, may lead to cell death or cancer. While there is considerable data on the repair of simple DSB (sDSB) by non-homologous end-joining (NHEJ), little is known about the repair of complex DSBs (cDSB), namely breaks with a nearby modification, which precludes ligation without prior processing. To study the mechanism of cDSB repair we developed a plasmid-based shuttle assay for the repair of a defined site-specific cDSB in cultured mammalian cells. Using this assay we found that repair efficiency and accuracy of a cDSB with an abasic site in a 5' overhang was reduced compared with a sDSB. Translesion DNA synthesis (TLS) across the abasic site located at the break prevented loss of DNA sequences, but was highly mutagenic also at the template base next to the abasic site. Similar to sDSB repair, cDSB repair was totally dependent on XrccIV, and altered in the absence of Ku80. In contrast, Artemis appears to be specifically involved in cDSB repair. These results may indicate that mammalian cells have a damage control strategy, whereby severe deletions are prevented at the expense of the less deleterious point mutations during NHEJ.

History

Publication status

  • Published

Journal

Nucleic Acids Research

ISSN

0305-1048

Publisher

Oxford University Press

Issue

20

Volume

37

Page range

6737-6745

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Notes

GDSC296

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2009-12-21

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