ATM and Artemis promote homologous recombination of radiation-induced DNA double-strand breaks in G2

Beucher, Andrea, Birraux, Julie, Tchouandong, Leopoldine, Barton, Olivia, Shibata, Atsushi, Conrad, Sandro, Goodarzi, Aaron A, Krempler, Andrea, Jeggo, Penny and Lo¨brich, Markus (2009) ATM and Artemis promote homologous recombination of radiation-induced DNA double-strand breaks in G2. EMBO Journal, 28 (21). pp. 3413-3427. ISSN 0261-4189

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Abstract

Homologous recombination (HR) and non-homologous end joining (NHEJ) represent distinct pathways for repairing DNA double-strand breaks (DSBs). Previous work implicated Artemis and ATM in an NHEJ-dependent process, which repairs a defined subset of radiation-induced DSBs in G1-phase. Here, we show that in G2, as in G1, NHEJ represents the major DSB-repair pathway whereas HR is only essential for repair of B15% of X- or c-rayinduced DSBs. In addition to requiring the known HR proteins, Brca2, Rad51 and Rad54, repair of radiation- induced DSBs by HR in G2 also involves Artemis and ATM suggesting that they promote NHEJ during G1 but HR during G2. The dependency for ATM for repair is relieved by depleting KAP-1, providing evidence that HR in G2 repairs heterochromatin-associated DSBs. Although not core HR proteins, ATM and Artemis are required for efficient formation of single-stranded DNA and Rad51 foci at radiation-induced DSBs in G2 with Artemis function
requiring its endonuclease activity. We suggest that
Artemis endonuclease removes lesions or secondary structures, which inhibit end resection and preclude the completion of HR or NHEJ.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH0301 Biology
?? QH426 ??
Depositing User: Gee Wheatley
Date Deposited: 20 Nov 2009
Last Modified: 07 Mar 2017 07:48
URI: http://sro.sussex.ac.uk/id/eprint/2262
Google Scholar:73 Citations

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