Schizosaccharomyces pombe Cds1 regulates homologous recombination at stalled replication forks through the phosphorylation of recombination protein Rad60

Miyabe, Izumi, Morishita, Takashi, Shinagawa, Hideo and Carr, Antony M (2009) Schizosaccharomyces pombe Cds1 regulates homologous recombination at stalled replication forks through the phosphorylation of recombination protein Rad60. Journal of Cell Science, 122. pp. 3638-3643. ISSN 0021-9533

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Abstract

The Schizosaccharomyces pombe rad60 gene is essential for cell growth and is involved in repairing DNA double-strand breaks. Rad60 physically interacts with, and is functionally related to, the structural maintenance of chromosomes 5 and 6 protein complex (Smc5/6). Rad60 is phosphorylated in response to hydroxyurea (HU)-induced DNA replication arrest in a Cds1Chk2- dependent manner. Rad60 localizes in nucleus in unchallenged cells, but becomes diffused throughout the cell in response to HU. To understand the role of Rad60 phosphorylation, we mutated the putative phosphorylation target motifs of Cds1Chk2 and have identified two Cds1Chk2 target residues responsible for Rad60 dispersal in response to HU. We show that the phosphorylationdefective rad60 mutation partially suppresses HU sensitivity and the elevated recombination frequency of smc6-X. Our data suggest that Rad60 phosphorylation is required to regulate homologous recombination at stalled replication forks, probably by regulating Smc5/6. Supplementary material available online at http://jcs.biologists.org/cgi/content/full/122/20/3638/DC1

Item Type: Article
Additional Information: GDSC294
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Gee Wheatley
Date Deposited: 18 Sep 2009
Last Modified: 20 Jun 2013 15:19
URI: http://sro.sussex.ac.uk/id/eprint/2246
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