The synaptonemal complex protein, Zip1 promotes the segregation of nonexchange chromosomes at meiosis I

Newnham, Louise, Jordan, Philip, Rockmill, Beth, Roeder, G Shirleen and Hoffmann, Eva (2010) The synaptonemal complex protein, Zip1 promotes the segregation of nonexchange chromosomes at meiosis I. Proceedings of the National Academy of Sciences, 107 (2). pp. 781-785. ISSN 1091-6490

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Abstract

Crossing over establishes connections between homologous chromosomes that promote their proper segregation at the first meiotic division. However, there exists a backup system to ensure the correct segregation of those chromosome pairs that fail to cross over. We have found that, in budding yeast, a mutation eliminating the synaptonemal complex protein, Zip1, increases the meiosis I nondisjunction rate of nonexchange chromosomes (NECs). The centromeres of NECs become tethered during meiotic prophase, and this tethering is disrupted by the zip1 mutation. Furthermore, the Zip1 protein often colocalizes to the centromeres of the tethered chromosomes, suggesting that Zip1 plays a direct role in holding NECs together. Zip3, a protein involved in the initiation of synaptonemal complex formation, is also important for NEC segregation. In the absence of Zip3, both the tethering of NECs and the localization of Zip1 to centromeres are impaired. A mutation in the MAD3 gene, which encodes a component of the spindle checkpoint, also increases the nondisjunction of NECs. Together, the zip1 and mad3 mutations have an additive effect, suggesting that these proteins act in parallel pathways to promote NEC segregation. We propose that Mad3 promotes the segregation of NECs that are not tethered by Zip1 at their centromeres.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: Q Science
Depositing User: Louise Newnham
Date Deposited: 06 Feb 2012 19:50
Last Modified: 26 Jun 2013 10:46
URI: http://sro.sussex.ac.uk/id/eprint/22426
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