Evidence that the extracytoplasmic function sigma factor s(E) is required for normal cell wall structure in Streptomyces coelicolor A3(2)

Paget, Mark S B, Chamberlin, Leony, Atrih, Abdelmadjid, Foster, Simon J and Buttner, Mark J (1999) Evidence that the extracytoplasmic function sigma factor s(E) is required for normal cell wall structure in Streptomyces coelicolor A3(2). Journal of Bacteriology, 181 (1). pp. 204-211. ISSN 00219193

Full text not available from this repository.

Abstract

The sigE gene of Streptomyces coelicolor A3(2) encodes an RNA polymerase sigma factor belonging to the extracytoplasmic function (ECF) subfamily. Constructed sigE deletion and disruption mutants were more sensitive than the parent to muramidases such as hen egg white lysozyme and to the CwlA amidase from Bacillus subtilis. This correlated with an altered muropeptide profile, as determined by reverse-phase high-performance liquid chromatography analysis of lytic digests of purified peptidoglycan. The sigE mutants required high levels of magnesium for normal growth and sporulation, overproducing the antibiotic actino-rhodin and forming crenellated colonies in its absence. Together, these data suggest that sigE is required for normal cell wall structure. The role of s(E) was further investigated by analyzing the expression of hrdD, which is partially sigE dependent. The hrdD gene, which encodes the s(HrdD) subunit of RNA polymerase, is transcribed from two promoters, hrdDp1 and hrdDp2, both similar to promoters recognized by other ECF sigma factors. The activities of hrdDp1 and hrdDp2 were reduced 20- and 3-fold, respectively, in sigE mutants, although only hrdDp1 was recognized by Es(E) in vitro. Growth on media deficient in magnesium caused the induction of both hrdD promoters in a sigE-dependent manner

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Depositing User: Mark Paget
Date Deposited: 06 Feb 2012 19:49
Last Modified: 21 Mar 2012 15:12
URI: http://sro.sussex.ac.uk/id/eprint/22326
📧 Request an update