Refolding of Ribonuclease A monitored by real-time photo-CIDNP NMR spectroscopy

Day, Iain J, Maeda, Kiminori, Paisley, Howard J, Mok, K Hun and Hore, P J (2009) Refolding of Ribonuclease A monitored by real-time photo-CIDNP NMR spectroscopy. Journal of Biomolecular NMR, 44 (2). pp. 77-86. ISSN 1573-5001

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Abstract

Photo-CIDNP NMR spectroscopy is a powerful method for investigating the solvent accessibility of histi- dine, tyrosine and tryptophan residues in a protein. When coupled to real-time NMR, this technique allows changes in the environments of these residues to be used as a probe of protein folding. In this paper we describe experiments performed to monitor the refolding of ribonuclease A fol- lowing dilution from a high concentration of chemical denaturant. These experiments provide a good example of the utility of this technique which provides information that is difficult to obtain by other biophysical methods. Real- time photo-CIDNP measurements yield residue-specific kinetic data pertaining to the folding reaction, interpreted in terms of current knowledge of the folding of bovine pancreatic ribonuclease A.

Item Type: Article
Schools and Departments: School of Life Sciences > Chemistry
Depositing User: Iain Day
Date Deposited: 06 Feb 2012 19:48
Last Modified: 30 Nov 2012 17:04
URI: http://sro.sussex.ac.uk/id/eprint/22265
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