Two clades of north European pool frogs Rana lessonae identified by cytochrome b sequence analysis

Zeisset, Inga and Beebee, Trevor J C (2007) Two clades of north European pool frogs Rana lessonae identified by cytochrome b sequence analysis. Herpetological Journal, 17 (4). pp. 255-260. ISSN 0268-0130

Full text not available from this repository.

Abstract

A 410 base-pair region of the mitochondrial cytochrome b (cytb) gene, including the coding region for amino acids 44-179, was amplified and sequenced from a total of 53 pool frogs (Rana lessonae) sampled in nine European countries across the species' biogeographical range. Just two haplotypes were found, differing by single base pair (G-A transition) at a codon second position, corresponding to a conservative (serine-asparagine) change at amino acid 75. Only haplotype A (serine 75) was found in the samples from Italy, Hungary, Switzerland, Poland, Sweden, Norway and Britain (including museum specimens). Only haplotype B (asparagine 75) was found in France, and there were equal numbers of A and B haplotypes in a small sample from the Netherlands. Comparisons with other ranid cytb sequences together with an absence of stop codons indicated that the sequences were mitochondrial rather than nuclear copies. Using these north European cytb sequences and three more from other work based on individuals from Italy, Ukraine and Luxembourg, we discuss haplotype distribution with respect to the phylogeography of R. lessonae.

Item Type: Article
Schools and Departments: School of Life Sciences > Evolution, Behaviour and Environment
Depositing User: Inga Zeisset
Date Deposited: 06 Feb 2012 19:42
Last Modified: 25 Apr 2012 09:21
URI: http://sro.sussex.ac.uk/id/eprint/21814
📧 Request an update