Regulation of ribonucleotide reductase by Spd1 involves multiple mechanisms

Nestoras, Konstantinos, Mohammed, Asma Hadi, Schreurs, Ann-Sofie, Fleck, Oliver, Watson, Adam T., Poitelea, Marius, O'Shea, Charlotte, Chacwan, Charly, Holmberg, Christian, Kragelund, Birthe B, Nielsen, Olaf, Osborne, Mark, Carr, Antony M. and Liu, Cong (2010) Regulation of ribonucleotide reductase by Spd1 involves multiple mechanisms. Genes and Development, 24 (11). pp. 1145-1159. ISSN 0890-9369

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Abstract

The correct levels of deoxyribonucleotide triphosphates and their relative abundance are important to maintain genomic integrity. Ribonucleotide reductase (RNR) regulation is complex and multifaceted. RNR is regulated allosterically by two nucleotide-binding sites, by transcriptional control, and by small inhibitory proteins that associate with the R1 catalytic subunit. In addition, the subcellular localization of the R2 subunit is regulated through the cell cycle and in response to DNA damage. We show that the fission yeast small RNR inhibitor Spd1 is intrinsically disordered and regulates R2 nuclear import, as predicted by its relationship to Saccharomyces cerevisiae Dif1. We demonstrate that Spd1 can interact with both R1 and R2, and show that the major restraint of RNR in vivo by Spd1 is unrelated to R2 subcellular localization. Finally, we identify a new behavior for RNR complexes that potentially provides yet another mechanism to regulate dNTP synthesis via modulation of RNR complex architecture.

Item Type: Article
Schools and Departments: School of Life Sciences > Chemistry
Subjects: Q Science
Depositing User: Konstantinos Nestoras
Date Deposited: 06 Feb 2012 19:32
Last Modified: 03 Oct 2012 11:27
URI: http://sro.sussex.ac.uk/id/eprint/21171
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