Smc5/6 maintains stalled replication forks in a recombination-competent conformation

Irmisch, Anja, Ampatzidou, Eleni, Mizuno, Ken'ichi, O'Connell, Matthew J and Murray, Johanne M (2009) Smc5/6 maintains stalled replication forks in a recombination-competent conformation. EMBO Journal, 28 (2). pp. 144-155. ISSN 0261-4189

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Abstract

The Smc5/6 structural maintenance of chromosomes complex is required for efficient homologous recombination (HR). Defects in Smc5/6 result in chromosome missegregation and fragmentation. By characterising two Schizosaccharomyces pombe smc6 mutants, we define two separate functions for Smc5/6 in HR. The first represents the previously described defect in processing recombination-dependent DNA intermediates when replication forks collapse, which leads to increased rDNA recombination. The second novel function defines Smc5/6 as a positive regulator of recombination in the rDNA and correlates mechanistically with a requirement to load RPA and Rad52 onto chromatin genome-wide when replication forks are stably stalled by nucleotide depletion. Rad52 is required for all HR repair, but Rad52 loading in response to replication fork stalling is unexpected and does not correlate with damage-induced foci. We propose that Smc5/6 is required to maintain stalled forks in a stable recombination-competent conformation primed for replication restart.

Item Type: Article
Additional Information: On Endnotes Ref: GDSC271
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
Depositing User: Gee Wheatley
Date Deposited: 23 Feb 2009
Last Modified: 23 Mar 2017 05:34
URI: http://sro.sussex.ac.uk/id/eprint/2117
Google Scholar:17 Citations

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