The Zipper Region of Epstein-Barr Virus bZIP Transcription Factor Zta Is Necessary but Not Sufficient To Direct DNA Binding

Hicks, Matthew R., Al-Mehairi, Salama S. and Sinclair, Alison J. (2003) The Zipper Region of Epstein-Barr Virus bZIP Transcription Factor Zta Is Necessary but Not Sufficient To Direct DNA Binding. Journal of Virology, 77 (14). pp. 8173-8177. ISSN 0022-538X

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Abstract

The viral bZIP transcription factor Zta (BZLF1, EB1, ZEBRA) mediates the switch between the latent and lytic cycles of Epstein-Barr virus (EBV). In part, its activity requires the formation of homodimers and interaction with specific DNA sequence elements (ZREs). Zta has an atypical zipper motif that has a lower stability than do typical bZIP proteins. Here we show that a synthetic peptide directed against the zipper can disrupt the DNA-binding function of Zta. This highlights the relevance of this region for the function of Zta and demonstrates that the zipper region is a potential target for therapeutic agents. We also unmask the relevance of a region adjacent to the zipper (CT region), which is required to direct the interaction of Zta with DNA and to transactivate ZRE-dependent promoters in vivo.

Item Type: Article
Additional Information: This research was entirely undertaken in Dr Sinclair's group at the University of Sussex. The work identifies the functional relevance of a novel region of a viral transcription factor.
Schools and Departments: School of Life Sciences > Biochemistry
Depositing User: Alison Sinclair
Date Deposited: 06 Feb 2012 19:30
Last Modified: 30 Mar 2012 14:59
URI: http://sro.sussex.ac.uk/id/eprint/20955
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