Distance measurements in the borderline region of applicability of CW EPR and DEER: A model study on a homologous series of spin-labelled peptides

Banham, J E, Baker, C M, Ceola, S, Day, I J, Grant, G H, Groenen, E J J, Rodgers, C T, Jeschke, G and Timmel, C R (2008) Distance measurements in the borderline region of applicability of CW EPR and DEER: A model study on a homologous series of spin-labelled peptides. Journal of Magnetic Resonance, 191 (2). pp. 202-218. ISSN 1090-7807

Full text not available from this repository.

Abstract

Inter-spin distances between 1 nm and 4.5 nm are measured by continuous wave (CW) and pulsed electron paramagnetic resonance (EPR) methods for a series of nitroxide-spin-labelled peptides. The upper distance limit for measuring dipolar coupling by the broad- ening of the CW spectrum and the lower distance limit for the present optimally-adjusted double electron electron resonance (DEER) set-up are determined and found to be both around 1.61.9 nm. The methods for determining distances and corresponding distributions from CW spectral line broadening are reviewed and further developed. Also, the work shows that a correction factor is required for the analysis of inter-spin distances below approximately 2 nm for DEER measurements and this is calculated using the density matrix formalism.

Item Type: Article
Schools and Departments: School of Life Sciences > Chemistry
Depositing User: Iain Day
Date Deposited: 06 Feb 2012 19:30
Last Modified: 30 Nov 2012 17:03
URI: http://sro.sussex.ac.uk/id/eprint/20938
📧 Request an update