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pEg7, a new xenopus protein required for mitotic chromosome condensation in egg extracts

journal contribution
posted on 2023-06-07, 23:00 authored by Fabien Cubizolles, Vincent Legagneux, René Le Guellec, Isabelle Chartrain, Rustem Uzbekov, Chris Ford, Katherine Le Guellec
We have isolated a cDNA, Eg7, corresponding to a Xenopus maternal mRNA, which is polyadenylated in mature oocytes and deadenylated in early embryos. This maternal mRNA encodes a protein, pEg7, whose expression is strongly increased during oocyte maturation. The tissue and cell expression pattern of pEg7 indicates that this protein is only readily detected in cultured cells and germ cells. Immunolocalization in Xenopus cultured cells indicates that pEg7 concentrates onto chromosomes during mitosis. A similar localization of pEg7 is observed when sperm chromatin is allowed to form mitotic chromosomes in cytostatic factor-arrested egg extracts. Incubating these extracts with antibodies directed against two distinct parts of pEg7 provokes a strong inhibition of the condensation and resolution of mitotic chromosomes. Biochemical experiments show that pEg7 associates with Xenopus chromosome-associated polypeptides C and E, two components of the 13S condensin.

History

Publication status

  • Published

Journal

Journal of Cell Biology

ISSN

0021-9525

Publisher

Rockefeller University Press

Issue

6

Volume

143

Page range

1437-1446

ISBN

0021-9525

Department affiliated with

  • Biology and Environmental Science Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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