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Early replication of short telomeres in budding yeast
journal contribution
posted on 2023-06-07, 14:51 authored by Alessandro BianchiAlessandro Bianchi, David ShoreThe maintenance of an appropriate number of telomere repeats by telomerase is essential for proper chromosome protection. The action of telomerase at the telomere terminus is regulated by opposing activities that either recruit/activate the enzyme at shorter telomeres or inhibit it at longer ones, thus achieving a stable average telomere length. To elucidate the mechanistic details of telomerase regulation we engineered specific chromosome ends in yeast so that a single telomere could be suddenly shortened and, as a consequence of its reduced length, elongated by telomerase. We show that shortened telomeres replicate early in S phase, unlike normal-length telomeres, due to the early firing of origins of DNA replication in subtelomeric regions. Early telomere replication correlates with increased telomere length and telomerase activity. These data reveal an epigenetic effect of telomere length on the activity of nearby replication origins and an unanticipated link between telomere replication timing and telomerase action.
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Publication status
- Published
File Version
- Published version
Journal
CellISSN
0092-8674Publisher
ElsevierExternal DOI
Issue
6Volume
128Page range
1051-1062Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2008-10-20First Compliant Deposit (FCD) Date
2018-04-13Usage metrics
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