University of Sussex
Browse

File(s) not publicly available

Measurement of chromosomal DNA single-strand breaks and replication fork progression rates

journal contribution
posted on 2023-06-07, 22:10 authored by Claire Breslin, Paula M Clements, Sherif F El-Khamisy, Eva Petermann, Natasha Iles, Keith CaldecottKeith Caldecott
Chromosomal single-strand breaks (SSBs) are the most common lesions arising in cells, but are normally rapidly repaired by multiprotein complexes centered around the scaffold protein, XRCC1. Here, we describe protocols to measure chromosomal SSBs in cells and for recovering and identifying novel components of SSBR complexes in vitro and in vivo. We also describe an assay we employ to measure the rate of replication fork progression in mammalian/vertebrate cells in the presence or absence of DNA damage.

History

Publication status

  • Published

Journal

Methods in Enzymology

ISSN

0076-6879

Volume

409

Page range

410-425

Pages

16.0

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC