Measurement of chromosomal DNA single-strand breaks and replication fork progression rates

Breslin, Claire, Clements, Paula M, El-Khamisy, Sherif F, Petermann, Eva, Iles, Natasha and Caldecott, Keith W (2006) Measurement of chromosomal DNA single-strand breaks and replication fork progression rates. Methods in Enzymology, 409. pp. 410-425. ISSN 0076-6879

Full text not available from this repository.

Abstract

Chromosomal single-strand breaks (SSBs) are the most common lesions arising in cells, but are normally rapidly repaired by multiprotein complexes centered around the scaffold protein, XRCC1. Here, we describe protocols to measure chromosomal SSBs in cells and for recovering and identifying novel components of SSBR complexes in vitro and in vivo. We also describe an assay we employ to measure the rate of replication fork progression in mammalian/vertebrate cells in the presence or absence of DNA damage.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Claire Breslin
Date Deposited: 06 Feb 2012 19:10
Last Modified: 30 Nov 2012 17:02
URI: http://sro.sussex.ac.uk/id/eprint/19502
📧 Request an update