Poly(ADP-Ribose) Polymerase 1 Accelerates Single-Strand Break Repair in Concert with Poly(ADP-Ribose) Glycohydrolase

Fisher, Anna E O, Hochegger, Helfrid, Takeda, Shunichi and Caldecott, Keith W (2007) Poly(ADP-Ribose) Polymerase 1 Accelerates Single-Strand Break Repair in Concert with Poly(ADP-Ribose) Glycohydrolase. Molecular and Cellular Biology, 27 (15). pp. 5597-5605. ISSN 0270-7306

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Abstract

Single-strand breaks are the commonest lesions arising in cells, and defects in their repair are implicated in neurodegenerative disease. One of the earliest events during single-strand break repair (SSBR) is the rapid synthesis of poly(ADP-ribose) (PAR) by poly(ADP-ribose) polymerase (PARP), followed by its rapid degradation by poly(ADP-ribose) glycohydrolase (PARG). While the synthesis of poly(ADP-ribose) is important for rapid rates of chromosomal SSBR, the relative importance of poly(ADP-ribose) polymerase 1 (PARP-1) and PARP-2 and of the subsequent degradation of PAR by PARG is unclear. Here we have quantified SSBR rates in human A549 cells depleted of PARP-1, PARP-2, and PARG, both separately and in combination. We report that whereas PARP-1 is critical for rapid global rates of SSBR in human A549 cells, depletion of PARP-2 has only a minor impact, even in the presence of depleted levels of PARP-1. Moreover, we identify PARG as a novel and critical component of SSBR that accelerates this process in concert with PARP-1.

Item Type: Article
Additional Information: From SRO
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Helfrid Hochegger
Date Deposited: 06 Feb 2012 19:10
Last Modified: 07 Mar 2017 06:18
URI: http://sro.sussex.ac.uk/id/eprint/19496

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