LAMMER kinase Kic1 is involved in pre-mRNA processing

Tang, Zhaohua, Luca, Maria, Portillio, Jessica, Ngo, Benson, Chang, Cathey, Wen, Teresa, Murray, Johanne and Carr, Antony (2011) LAMMER kinase Kic1 is involved in pre-mRNA processing. Experimental Cell Research, 317 (16). pp. 2308-2320. ISSN 0014-4827

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Abstract

The LAMMER kinases are conserved through evolution. They play vital roles in cell growth/differentiation, development, and metabolism. One of the best known functions of the kinases in animal cells is the regulation of pre-mRNA splicing. Kic1 is the LAMMER kinase in fission yeast Schizosaccharomyces pombe. Despite the reported pleiotropic effects of kic1+ deletion/overexpression on various cellular processes the involvement of Kic1 in splicing remains elusive. In this study, we demonstrate for the first time that Kic1 not only is required for efficient splicing but also affects mRNA export, providing evidence for the conserved roles of LAMMER kinases in the unicellular context of fission yeast. Consistent with the hypothesis of its direct participation in multiple steps of pre-mRNA processing, Kic1 is predominantly present in the nucleus during interphase. In addition, the kinase activity of Kic1 plays a role in modulating its own cellular partitioning. Interestingly, Kic1 expression oscillates in a cell cycle-dependent manner and the peak level coincides with mitosis and cytokinesis, revealing a potential mechanism for controlling the kinase activity during the cell cycle. The novel information about the in vivo functions and regulation of Kic1 offers insights into the conserved biological roles fundamental to LAMMER kinases in eukaryotes.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Johanne Murray
Date Deposited: 06 Feb 2012 19:03
Last Modified: 15 Jun 2012 10:39
URI: http://sro.sussex.ac.uk/id/eprint/19204
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