Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry.

Sweet, Steve, Bailey, C.M, Cunningham, D.L., Heath, J.K and Cooper, H.J. (2009) Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry. Molecular and Cellular Proteomics, 8 (5). pp. 904-912. ISSN 15359476

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Abstract

We used on-line electron capture dissociation (ECD) for the large scale identification and localization of sites of phosphorylation. Each FT-ICR ECD event was paired with a linear ion trap collision-induced dissociation (CID) event, allowing a direct comparison of the relative merits of ECD and CID for phosphopeptide identification and site localization. Linear ion trap CID was shown to be most efficient for phosphopeptide identification, whereas FT-ICR ECD was superior for localization of sites of phosphorylation. The combination of confident CID and ECD identification and confident CID and ECD localization is particularly valuable in cases where a phosphopeptide is identified just once within a phosphoproteomics experiment. © 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: Q Science
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Depositing User: Steve Sweet
Date Deposited: 06 Feb 2012 19:01
Last Modified: 15 Oct 2012 13:53
URI: http://sro.sussex.ac.uk/id/eprint/19005
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