Identification of the Hair Cell Soma-1 Antigen, HCS-1, as Otoferlin

Goodyear, Richard J, Legan, P Kevin, Christiansen, Jeffrey R, Xia, Bei, Korchagina, Julia, Gale, Jonathan E, Warchol, Mark E, Corwin, Jeffrey T and Richardson, Guy P (2010) Identification of the Hair Cell Soma-1 Antigen, HCS-1, as Otoferlin. Journal of the Association for Research in Otolaryngology, 11 (4). pp. 573-586. ISSN 1525-3961

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Hair cells, the mechanosensitive receptor cells of the inner ear, are critical for our senses of hearing and balance. The small number of these receptor cells in the inner ear has impeded the identification and characterization of proteins important for hair cell function. The binding specificity of monoclonal antibodies provides a means for identifying hair cell-specific proteins and isolating them for further study. We have generated a monoclonal antibody, termed hair cell soma-1 (HCS-1), which specifically immunolabels hair cells in at least five vertebrate classes, including sharks and rays, bony fish, amphibians, birds, and mammals. We used HCS-1 to immunoprecipitate the cognate antigen and identified it as otoferlin, a member of the ferlin protein family. Mutations in otoferlin underlie DFNB9, a recessive, nonsyndromic form of prelingual deafness characterized as an auditory neuropathy. Using immunocytochemistry, we find that otoferlin is associated with the entire basolateral membrane of the hair cells and with vesicular structures distributed throughout most of the hair cell cytoplasm. Biochemical assays indicate that otoferlin is tightly associated with membranes, as it is not solubilized by alterations in calcium or salt concentrations. HCS-1 immunolabeling does not co-localize with ribeye, a constituent of synaptic ribbons, suggesting that otoferlin may, in addition to its proposed function in synaptic vesicle release, play additional roles in hair cells.

Item Type: Article
Schools and Departments: School of Life Sciences > Neuroscience
Depositing User: Richard Goodyear
Date Deposited: 06 Feb 2012 18:51
Last Modified: 30 Nov 2012 17:01
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