The Bre5/Ubp3 ubiquitin protease complex from budding yeast contributes to the cellular response to DNA damage.

Bilsland, Elizabeth, Hult, Malin, Bell, Stephen D, Sunnerhagen, Per and Downs, Jessica A (2007) The Bre5/Ubp3 ubiquitin protease complex from budding yeast contributes to the cellular response to DNA damage. DNA Repair, 6 (10). pp. 1471-1484. ISSN 1568-7864

Full text not available from this repository.

Abstract

The ubiquitination status of proteins can control numerous aspects of protein function through targeted destruction or by altering protein¿protein interactions, subcellular localization, or enzymatic activity. In addition to enzymes that mediate the conjugation of ubiquitin moieties to target proteins, there are enzymes that catalyze the removal of ubiquitin, termed ubiquitin proteases. One such ubiquitin protease, Ubp3, exists in a complex with a partner protein: Bre5. This complex has been implicated in a variety of cellular activities, and was recently identified in large-scale screens for genetic interactions with known components of the DNA damage response pathway. We found that this complex plays a role in the cellular response to the DNA damaging agent phleomycin and strains lacking the complex have a defect in non-homologous end joining. Although this complex is also important for telomeric silencing, maintenance of the cell wall, and global transcriptional regulation, we present evidence suggesting that the role of this complex in DNA damage responses is distinct from these other roles. First, we found that Ubp3/Bre5 functions antagonistically with Bul1 in DNA damage responses, but not in its other cellular functions. Additionally, we have generated mutants of Bre5 that are specifically defective in DNA damage responses

Item Type: Article
Additional Information: This project was designed and orchestrated by JD and carried out in the JD lab, with limited experimental input from collaborators. JD prepared the manuscript.
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Jessica Downs
Date Deposited: 06 Feb 2012 18:44
Last Modified: 29 Mar 2012 14:03
URI: http://sro.sussex.ac.uk/id/eprint/18104
📧 Request an update