Photo-CIDNP NMR spectroscopy of a heme-containing protein

Day, Iain J., Wain, Rachel, Tozawa, Kaeko, Smith, Lorna J. and Hore, P. J. (2005) Photo-CIDNP NMR spectroscopy of a heme-containing protein. Journal of Magnetic Resonance, 175 (2). pp. 330-335. ISSN 1090-7807

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Abstract

There are relatively few examples of the application of photo-CIDNP NMR spectroscopy to chromophore-containing proteins. The most likely reason for this is that simultaneous absorption of light by the photosensitiser molecule and the protein chromophore reduces the effectiveness of the photochemical reaction that produces the observed nuclear polarisation. We present details of exper- iments performed on the air-oxidised form of a small cytochrome, from the thermophilic bacterium Hydrogenobacter thermophilus, using both the wild-type protein and apo and holo forms of a double alanine b-type mutant. We show that, along with the apo state, it is possible to generate CIDNP in the air-oxidised form of the b-type mutant, but not in the corresponding c-type cytochrome. This finding is supported by control experiments using horse-heart cytochrome c.

Item Type: Article
Schools and Departments: School of Life Sciences > Chemistry
Depositing User: Iain Day
Date Deposited: 06 Feb 2012 18:42
Last Modified: 22 Mar 2012 15:28
URI: http://sro.sussex.ac.uk/id/eprint/17833
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