Arginine and methylated arginines in human plasma and urine measured by tandem mass spectrometry without the need for chromatography or sample derivatisation

Weaving, Gary, Rocks, Bernard F, Bailey, Michael P and Titheradge, Michael A (2008) Arginine and methylated arginines in human plasma and urine measured by tandem mass spectrometry without the need for chromatography or sample derivatisation. Journal of Chromatography B, 874 (1-2). pp. 27-32. ISSN 1570-0232

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Abstract

A method for the simultaneous analysis of asymmetric dimethylarginine, symmetric dimethylarginine, monomethylarginine and arginine in human plasma and urine, with short analysis time and isotopic internal standardisation for each analyte is described. The method requires neither sample derivatisation nor the need for chromatographic separation of analytes. The method described shows good precision and accuracy and is suited for both research purposes and implementation in the busy, routine clinical laboratory. In addition the synthesis and utilisation of isotopically labelled symmetric dimethylarginine and monomethylarginine is described for the first time, avoiding the use of surrogates such as homoarginine for internal standardisation.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Brighton and Sussex Medical School > Division of Medical Education
Depositing User: Gary Ronald Weaving
Date Deposited: 06 Feb 2012 18:34
Last Modified: 22 Sep 2017 13:11
URI: http://sro.sussex.ac.uk/id/eprint/17166
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