The effect of elevated concentrations of fructose 2,6-bisphosphate on carbon metabolism during deacidification in the crassulacean acid metabolism plant Kalanchöe daigremontiana

Truesdale, Mark R, Toldi, Otto and Scott, Peter (1999) The effect of elevated concentrations of fructose 2,6-bisphosphate on carbon metabolism during deacidification in the crassulacean acid metabolism plant Kalanchöe daigremontiana. Plant Physiology, 121 (3). pp. 957-964. ISSN 0032-0889

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Abstract

In C3 plants, the metabolite fructose 2,6-bisphosphate (Fru 2,6-P2) has an important role in the regulation of carbon partitioning during photosynthesis. To investigate the impact of Fru 2,6-P2 on carbon metabolism during Crassulacean acid metabolism (CAM), we have developed an Agrobacterium tumefaciens-mediated transformation system in order to alter genetically the obligate CAM plant Kalanchöe daigremontiana. To our knowledge, this is the first report to use genetic manipulation of a CAM species to increase our understanding of this important form of plant metabolism. Transgenic plants were generated containing a modified rat liver 6-phosphofructo-2-kinase gene. In the plants analyzed the activity of 6-phosphofructo-2-kinase ranged from 175% to 198% of that observed in wild-type plants, resulting in Fru 2,6-P2 concentrations that were 228% to 350% of wild-type plants after 2 h of illumination. A range of metabolic measurements were made on these transgenic plants to investigate the possible roles of Fru 2,6-P2 during Suc, starch, and malic acid metabolism across the deacidification period of CAM. The results suggest that Fru 2,6-P2 plays a major role in regulating partitioning between Suc and starch synthesis during photosynthesis. However, alterations in Fru 2,6-P2 levels had little effect on malate mobilization during CAM fluxes.

Item Type: Article
Schools and Departments: School of Life Sciences > Biology and Environmental Science
Depositing User: Peter Scott
Date Deposited: 06 Feb 2012 18:32
Last Modified: 14 Sep 2012 13:25
URI: http://sro.sussex.ac.uk/id/eprint/17023
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