The C-terminal domain of eukaryotic protein synthesis initiation factor (eIF4G is sufficient to support cap-independent translation in the absence ofeIF4E

Ohlmann, T, Rau, M, Pain, V M and Morley, S J (1996) The C-terminal domain of eukaryotic protein synthesis initiation factor (eIF4G is sufficient to support cap-independent translation in the absence ofeIF4E. EMBO Journal, 15 (6). pp. 1371-1382. ISSN 0261-4189

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Abstract

The foot and mouth disease virus, a picornavirus, encodes two forms of a cysteine proteinase (leader or L protease) that bisects the eIF4G polypeptide of the initiation factor complex eIF4F into N-terminal (N(t)) and C-terminal (C(t)) domains. Previously we showed that, although in vitro cleavage of the translation initiation factor, eIF4G, with L protease decreases cap-dependent translation, the cleavage products themselves may directly promote cap-independent protein synthesis. We now demonstrate that translation of uncapped mRNAs normally exhibits a strong requirement for eIF4E. However, this dependence is abolished when eIF4G is cleaved, with the C(t) domain capable of supporting translation in the absence of the N(t) domain. In contrast, the efficient translation of the second cistron of bicistronic mRNAs, directed by two distinct Internal Ribosome Entry Segments (IRES), exhibits no requirement for eIF4E but is dependent upon either intact eIF4G or the C(t) domain. These results demonstrate that: (i) the apparent requirement for eIF4F for internal initiation on IRES-driven mRNAs can be fulfilled by the C(t) proteolytic cleavage product; (ii) when eIF4G is cleaved, the C(t) domain can also support cap-independent translation of cellular mRNAs not possessing an IRES element, in the absence of eIF4E; and (iii) when eIF4G is intact, translation of cellular mRNAs, whether capped or uncapped, is strictly dependent upon eIF4E. These data complement recent work in other laboratories defining the binding sites for other initiation factors on the eIF4G molecule.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Depositing User: Simon Morley
Date Deposited: 06 Feb 2012 18:27
Last Modified: 14 Jun 2012 09:01
URI: http://sro.sussex.ac.uk/id/eprint/16485
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