Conservation of cancer genes in the invertebrate, Mytilus edulis

Ciocan, Corina M and Rotchell, Jeanette M (2005) Conservation of cancer genes in the invertebrate, Mytilus edulis. Environmental Science and Technology, 39 (9). pp. 3029-3033. ISSN 0013-936X

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Abstract

Mussels are susceptible to a wide range of environmental toxicants, including carcinogens, and thus are often employed as bioindicator species. To elucidate the molecular aetiology of such neoplastic damage, we have cloned Mytilus edulis homologues of the vertebrate ras proto-oncogene, and p53 tumour suppressor gene. The M. edulis ras cDNA encodes a predicted protein of 184 amino acids. The DNA sequence analysis with vertebrate ras sequences demonstrates that the M. edulis ras cDNA is highly conserved in regions of functional importance, including mutational hot spots. The partial p53 sequence also demonstrates that M. edulis p53 is highly conserved in two regions of functional importance and that these regions also include four of the five mutational hot spots for this gene. In contrast, the M. edulis p53 sequence shows little similarity to the other published invertebrate p53-like sequences. The cancer gene sequences characterised herein will allow development of specific biomarkers of genotoxic damage.

Item Type: Article
Additional Information: Shellfish industries lose a third of stock each year to causes unknown. My post-doc, Ciocan, working entirely under my supervision, performed the lab-work and I co-ordinated the EU-funded project. The molecular approach adopted here was pioneering and has since been adopted by other groups.
Schools and Departments: School of Life Sciences > Biology and Environmental Science
Depositing User: Corina Ciocan
Date Deposited: 06 Feb 2012 18:21
Last Modified: 14 Jun 2012 11:16
URI: http://sro.sussex.ac.uk/id/eprint/15956
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