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A slow RNA polymerase II affects alternative splicing in vivo
journal contribution
posted on 2023-06-07, 19:37 authored by Manuel de la Mata, Claudio AlonsoClaudio Alonso, Sebastián Kadener, Juan P Fededa, Mati´as Blaustein, Federico Pelisch, Paula Cramer, David Bentley, Alberto R KornblihttChanges in promoter structure and occupation have been shown to modify the splicing pattern of several genes, evidencing a coupling between transcription and alternative splicing. It has been proposed that the promoter effect involves modulation of RNA pol II elongation rates. The C4 point mutation of the Drosophila pol II largest subunit confers on the enzyme a lower elongation rate. Here we show that expression of a human equivalent to Drosophila's C4 pol II in human cultured cells affects alternative splicing of the fibronectin EDI exon and adenovirus E1a pre-mRNA. Most importantly, resplicing of the Hox gene Ultrabithorax is stimulated in Drosophila embryos mutant for C4, which demonstrates the transcriptional control of alternative splicing on an endogenous gene. These results provide a direct proof for the elongation control of alternative splicing in vivo.
History
Publication status
- Published
Journal
Molecular CellISSN
1097-2765External DOI
Issue
2Volume
12Page range
525-532Pages
8.0Department affiliated with
- Evolution, Behaviour and Environment Publications
Notes
Initiated all the in vivo work, a contribution that gives this paper its present relevance (over 50 citations August 2007 see News and Views comment in Nature Structural and Molecular Biology 10), designed and executed all in vivo experiments, collected developmental and molecular data, analysed data, and co-wrote the paper.Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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