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A slow RNA polymerase II affects alternative splicing in vivo

journal contribution
posted on 2023-06-07, 19:37 authored by Manuel de la Mata, Claudio AlonsoClaudio Alonso, Sebastián Kadener, Juan P Fededa, Mati´as Blaustein, Federico Pelisch, Paula Cramer, David Bentley, Alberto R Kornblihtt
Changes in promoter structure and occupation have been shown to modify the splicing pattern of several genes, evidencing a coupling between transcription and alternative splicing. It has been proposed that the promoter effect involves modulation of RNA pol II elongation rates. The C4 point mutation of the Drosophila pol II largest subunit confers on the enzyme a lower elongation rate. Here we show that expression of a human equivalent to Drosophila's C4 pol II in human cultured cells affects alternative splicing of the fibronectin EDI exon and adenovirus E1a pre-mRNA. Most importantly, resplicing of the Hox gene Ultrabithorax is stimulated in Drosophila embryos mutant for C4, which demonstrates the transcriptional control of alternative splicing on an endogenous gene. These results provide a direct proof for the elongation control of alternative splicing in vivo.

History

Publication status

  • Published

Journal

Molecular Cell

ISSN

1097-2765

Issue

2

Volume

12

Page range

525-532

Pages

8.0

Department affiliated with

  • Evolution, Behaviour and Environment Publications

Notes

Initiated all the in vivo work, a contribution that gives this paper its present relevance (over 50 citations August 2007 see News and Views comment in Nature Structural and Molecular Biology 10), designed and executed all in vivo experiments, collected developmental and molecular data, analysed data, and co-wrote the paper.

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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