Double-stranded RNA interference shows that Engrailed controls the synaptic specificity of identified sensory neurons.

Marie, Bruno, Bacon, Jonathan P and Blagburn, Jonathan M (2000) Double-stranded RNA interference shows that Engrailed controls the synaptic specificity of identified sensory neurons. Current Biology, 10 (5). pp. 289-292. ISSN 09609822

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Abstract

The transcription factor Engrailed (En) controls the topography of axonal projections by regulating the expression of cell-adhesion molecules [1-4] but it is not known whether it also controls the choice of individual synaptic target cells. In the cercal sensory system of the larval cockroach (Periplaneta americana), small numbers of identified wind-sensitive sensory neurons form highly specific synaptic connections with 14 identified giant interneurons [5,6], and target-cell choice is independent of the pattern of axonal projections [6]. En is a putative positional determinant in the array of cercal sensory neurons [7]. In the present study, double-stranded RNA (dsRNA) interference [8] was used to abolish En expression. This treatment changed the axonal arborisation and synaptic outputs of an identified En-positive sensory neuron so that it came to resemble a nearby En-negative cell, which was itself unaffected. We thus demonstrate directly that En controls synaptic choice, as well as axon projections.

Item Type: Article
Additional Information: First use of dsRNA interference to knockout gene expression in identified neurons.
Schools and Departments: School of Life Sciences > Evolution, Behaviour and Environment
Depositing User: Jonathan Bacon
Date Deposited: 06 Feb 2012 18:17
Last Modified: 20 Mar 2012 10:00
URI: http://sro.sussex.ac.uk/id/eprint/15652
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