Aurora A and Aurora B jointly coordinate chromosome segregation

Hegarat, Nadia, Smith, Ewan, Nayak, Gowri, Takeda, Shunichi, Eyers, Patrick A and Hochegger, Helfrid (2011) Aurora A and Aurora B jointly coordinate chromosome segregation. Journal of Cell Biology, 195 (7). pp. 1103-1113. ISSN 0021-9525

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Abstract

We established a conditional deletion of Aurora A kinase (AurA) in Cdk1 analoguesensitive DT40 cells to analyze AurA knockout phenotypes after Cdk1 activation. In the absence of AurA, cells form bipolar spindles but fail to properly align their chromosomes and exit mitosis with segregation errors. The resulting daughter cells exhibit a variety of phenotypes and are highly aneuploid. Aurora B kinase (AurB)-inhibited cells show a similar chromosome alignment problem and cytokinesis defects, resulting in binucleate daughter cells. Conversely, cells lacking AurA and AurB activity exit mitosis without anaphase, forming polyploid daughter cells with a single nucleus. Strikingly, inhibition of both AurA and AurB results in a failure to depolymerize spindle microtubules (MTs) in anaphase after Cdk1 inactivation. These results suggest an essential combined function of AurA and AurB in chromosome segregation and anaphase MT dynamics.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: Q Science
Depositing User: Nadia Hegarat
Date Deposited: 18 Jul 2012 14:13
Last Modified: 22 Oct 2012 13:33
URI: http://sro.sussex.ac.uk/id/eprint/15123
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