Farah, J. A., Hartsuiker, E., Mizuno, K., Ohta, K. and Smith, G. R. (2002) A 160-bp palindrome is a Rad50.Rad32-dependent mitotic recombination hotspot in Schizosaccharomyces pombe. Genetics, 161 (1). pp. 461-8. ISSN 0016-6731Full text not available from this repository.
Palindromic sequences can form hairpin and cruciform structures that pose a threat to genome integrity. We found that a 160-bp palindrome (an inverted repeat of 80 bp) conferred a mitotic recombination hotspot relative to a control nonpalindromic sequence when inserted into the ade6 gene of Schizosaccharomyces pombe. The hotspot activity of the palindrome, but not the basal level of recombination, was abolished by a rad50 deletion, by a rad50S "separation of function" mutation, or by a rad32-D25A mutation in the nuclease domain of the Rad32 protein, an Mre11 homolog. We propose that upon extrusion of the palindrome the Rad50.Rad32 nuclease complex recognizes and cleaves the secondary structure thus formed and generates a recombinogenic break in the DNA.
|Additional Information:||0016-6731 Journal Article GDSC115|
|Keywords:||DNA-Binding Proteins/*metabolism Diploidy Fungal Proteins/*metabolism Haploidy Mitosis/genetics Plasmids/genetics *Recombination, Genetic *Repetitive Sequences, Nucleic Acid Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/genetics *Saccharomyces cerevisiae Proteins Schizosaccharomyces/*genetics|
|Schools and Departments:||School of Life Sciences|
|Subjects:||Q Science > QP Physiology|
|Depositing User:||Gee Wheatley|
|Date Deposited:||03 May 2007|
|Last Modified:||30 Nov 2012 16:51|
|Google Scholar:||53 Citations|