A 160-bp palindrome is a Rad50.Rad32-dependent mitotic recombination hotspot in Schizosaccharomyces pombe

Farah, J. A., Hartsuiker, E., Mizuno, K., Ohta, K. and Smith, G. R. (2002) A 160-bp palindrome is a Rad50.Rad32-dependent mitotic recombination hotspot in Schizosaccharomyces pombe. Genetics, 161 (1). pp. 461-8. ISSN 0016-6731

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Abstract

Palindromic sequences can form hairpin and cruciform structures that pose a threat to genome integrity. We found that a 160-bp palindrome (an inverted repeat of 80 bp) conferred a mitotic recombination hotspot relative to a control nonpalindromic sequence when inserted into the ade6 gene of Schizosaccharomyces pombe. The hotspot activity of the palindrome, but not the basal level of recombination, was abolished by a rad50 deletion, by a rad50S "separation of function" mutation, or by a rad32-D25A mutation in the nuclease domain of the Rad32 protein, an Mre11 homolog. We propose that upon extrusion of the palindrome the Rad50.Rad32 nuclease complex recognizes and cleaves the secondary structure thus formed and generates a recombinogenic break in the DNA.

Item Type: Article
Additional Information: 0016-6731 Journal Article GDSC115
Keywords: DNA-Binding Proteins/*metabolism Diploidy Fungal Proteins/*metabolism Haploidy Mitosis/genetics Plasmids/genetics *Recombination, Genetic *Repetitive Sequences, Nucleic Acid Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/genetics *Saccharomyces cerevisiae Proteins Schizosaccharomyces/*genetics
Schools and Departments: School of Life Sciences
Subjects: Q Science > QP Physiology
Depositing User: Gee Wheatley
Date Deposited: 03 May 2007
Last Modified: 30 Nov 2012 16:51
URI: http://sro.sussex.ac.uk/id/eprint/1057
Google Scholar:53 Citations
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