Expression, purification and preliminary X-ray analysis of the BRCT domain from Rhp9/Crb2

Hinks, J, Roe, Mark, Ho, J C, Watts, Felicity, Phelan, J, McAllister, M and Pearl, Laurence (2003) Expression, purification and preliminary X-ray analysis of the BRCT domain from Rhp9/Crb2. Acta Crystallographica Section D: Biological Crystallography, 59 (Pt 7). 1230-1233.. ISSN 0907-4449

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Abstract

The BRCT domain from Rhp9 (a Schizosaccharomyces pombe DNA-damage checkpoint protein) has been expressed, purified and crystallized. Overexpression in bacterial cells was achieved by minimizing aeration during host cell growth. A robotic screen was used to determine the solubility parameters; concentration of the protein was achieved by exploiting this information. Single crystals suitable for X-ray analysis were obtained in two forms by vapour diffusion (trigonal, unit-cell parameters a = b = 228.04, c = 70.42 A, and tetragonal, P4/m Laue group symmetry, unit-cell parameters a = b = 72.3, c = 91.1 A).

Item Type: Article
Additional Information: GDSC62
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Gee Wheatley
Date Deposited: 26 Mar 2007
Last Modified: 25 Jun 2015 10:52
URI: http://sro.sussex.ac.uk/id/eprint/1021
Google Scholar:2 Citations
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