Hinks, J, Roe, Mark, Ho, J C, Watts, Felicity, Phelan, J, McAllister, M and Pearl, Laurence (2003) Expression, purification and preliminary X-ray analysis of the BRCT domain from Rhp9/Crb2. Acta Crystallographica Section D: Biological Crystallography, 59 (Pt 7). 1230-1233.. ISSN 0907-4449Full text not available from this repository.
The BRCT domain from Rhp9 (a Schizosaccharomyces pombe DNA-damage checkpoint protein) has been expressed, purified and crystallized. Overexpression in bacterial cells was achieved by minimizing aeration during host cell growth. A robotic screen was used to determine the solubility parameters; concentration of the protein was achieved by exploiting this information. Single crystals suitable for X-ray analysis were obtained in two forms by vapour diffusion (trigonal, unit-cell parameters a = b = 228.04, c = 70.42 A, and tetragonal, P4/m Laue group symmetry, unit-cell parameters a = b = 72.3, c = 91.1 A).
|Schools and Departments:||School of Life Sciences > Sussex Centre for Genome Damage and Stability|
|Depositing User:||Gee Wheatley|
|Date Deposited:||26 Mar 2007|
|Last Modified:||25 Jun 2015 10:52|
|Google Scholar:||2 Citations|